山羊 FTO 基因克隆及其表达谱

doi:10.11843/j.issn.0366-6964.2016.05.005

Abstract

Abstract:

The aim of this study was to clarify the expression characteristics of FTO gene and investigate the correlation between FTO expression and intramuscular fat (IMF) content in muscles.The FTO gene was cloned from Jianzhou Da’er goat by RT-PCR method.Quantitative Real-time PCR(qPCR) and Western blotting were used to measure the expression profiles of FTO gene in tissues，especially in various parts of muscle of goats at different developmental stages.The correlation analysis was performed between FTO gene and IMF content.The results showed that goat FTO gene was 1 518 bp in CDS length，encoding 505 amino acids.The FTO protein was predicted to be non-secretory and lacking cross-membrane domain.The mRNA level of FTO was higher in adipose，spleen and lung(P＜0.01)，while lowest in longissimus dorsi；However，the protein level of FTO was higher in longissimus dorsi and spleen than that in adipose (P＜0.01).The mRNA level of FTO was significantly higher in longissimus dorsi and biceps femoris of 24-month-old goats than that in 1 to 3-month-old and 8 to 10-month-old goats (P＜0.05).The mRNA level of FTO was negatively correlated with IMF content of longissimus dorsi (P＜0.05) but positively correlated with IMF content of biceps femoris and triceps brachii.In this study，we investigated a significant correlation between FTO expression and IMF，which indicates that FTO may serve as a candidate gene for IMF.

CLC Number:

S827
S813.3

LIN Ya-qiu，LIAO Hong-hai，HE Qing-hua，LI Qian，WANG Yong. Cloning and Expression Profiling of FTO Gene of Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 888-898.

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Cloning and Expression Profiling of FTO Gene of Goat

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